Pyritum, one of the important prescriptions in the oriental medicine, has been used for conjugation fracture.
The purpose of this study is to evaluate the effects of administration of Pyritum on activation of osteoblast cells in human body and on tibia bone during early fracture healing phase in mice.
Four weeks aged 30 female DBA mice were used for this study. They are divided three groups, normal group, control group(Left tibia bones of mice were fractured by bone cutters) and experimental group(administration of Pyritum after fracture). Activation of osteoblast was identified using OPN mRNA, osteopontin in vitro test. In vivo test, regeneration of fractured tibia through the morphological changes was observed and the activation of proinflammatory cytokines (MIF, IL-6) in each group was measured.
1.In vitro test for activation of osteoblast cells in human body by Pyritum, OPN mRNA, osteopontin production were remarkably increased.
2.In activation of inflammation by Pyritum administered, activation of MIF, IL-6 were higher in the control and the experimental group than those of the normal group. Especially the experimental group showed higher activation and increase than those of the control group.
From the result, development of a new stimulator in healing fracture via pyrite is expected.